Beyond the
Last-Frame
Overview
Finding 02 / 05Investigation

Calibrating phasesto the data

The textbook says fluorescein angiography has three fixed phases. I tested that against the data — and it did not hold.

Clinical literature describes fluorescein angiography in three phases — early, mid and late — and a case report gave concrete boundaries at roughly 47.5 and 197.5 seconds. The tempting move is to take those numbers and move on.

But a convention from one clinical context is a hypothesis, not a fact, for this dataset. So it was tested — with a measurement instrument built specifically for the question.

The instrument

A one-dimensional LDA probe — cheap, and reusable.

A one-dimensional Linear Discriminant Analysis fitted on frozen embeddings, read through three metrics — balanced accuracy, distribution overlap, and the Fisher score. If a set of phase boundaries carves the data into genuinely distinct groups, the probe scores high. If the phases blur together, it scores low.

Textbook vs. data

The convention did not survive contact with the data.

Early
Mid
Late
0 s47.5 s197.5 selapsed →
Paper-derived boundaries (47.5 s / 197.5 s) — heavily skewed phase counts; balanced accuracy 0.643.
Early
Mid
Late
0 s103 s518 selapsed →
Data-driven boundaries (103 s / 518 s) — balanced phases; balanced accuracy 0.694. Carried downstream.
LD1 histogram under paper-derived phase boundaries
Fig. 5Under the textbook boundaries, the Early and Mid clusters sit almost on top of each other — only the Late phase is clearly displaced.

Phase boundaries should be calibrated to the data — not copied from a textbook.

The finding